Comparative analysis application of different cryoprotectors is in equilibration solution at cryopreservation of cows gametes

P. A. Trotskiy

The results of experimental researches on cryopreservation oocytes-cumulus complexes cows with the use of different cryoprotectors in equilibration solution and viability and subsequent development is appraised in vitro gametes after they were frozen-thawed are resulted in this article. It was established, that the use of ethylene glycol in equilibration solution at cryopreservation oocytes-cumulus complexes of cows is more effective comparatively with glycerol and propandiol. Ripening of cows frozen-thawed gametes out of organism was conducted. Morphological and cytogenetic analyses of ovules and obtained in vitro cattle embryos were also conducted.

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