Hypertonic cryohemolysis of mammalian erythrocytes in electrolyte and non-electrolyte media

N. M. Shpakova, S. S. Ershov, E. Ye. Nipot

There was studied the dependence of hypertonic cryohemolysis rate for mammalian erythrocytes on incubation time at 37 ?C and composition of hypertonic medium. The character of time dependences of hypertonic cryohemolysis for these cells has been shown to be determined by both erythrocyte's type and composition of hypertonic medium. It has been established that the dynamics of K+ ion release differs for the erythrocytes of different mammalian species in electrolyte and non-electrolyte hypertonic media and is not in the accordance with the rate of cell damage during cooling. It is supposed that K+ ion yield is not determining one for cell damage during cryohemolysis and the damage rate depends on membrane parameters of the cells under study.

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