The culture of embryos of domestic miniature fowl in the surrogated eggshells

A. Rudolf, M. W. Lis, K. Glodek, J. W. Niedziolka

The aim of this work was to comparison of chick embryo development of miniature hen and broiler cultures. At the experiment the Perry'88 culture embryo in surrogated eggshells with self-modification was used. The eggs of domestic miniature fowls and parental flock of Ross 308 broiler chicken were used as the donor. The recipient eggshells were taken out from the eggs of commercial layers.In the described experiment embryos of domestic miniature fowl and broiler chicken embryos cultured in surrogated eggshells showed differences in development, however none of embryos hatched. The first top of embryos mortalization was observed between stage S3 and S13 (25-30% of embryos) for miniature fowls while for broilers between S3 and S8 (23-35% of embryos). Next critical period was observed between S17 and S21. In this period 12-40% of miniature fowl embryos and 20% of broilers embryos finished development. In the described experiment the broiler chicken embryos were not able to develop longer than to 9th day of incubation and mortalized graduate between S25 - S35. The miniature fowl embryos characterized of better survival and some of them developed to 11th day of incubation (S37). However, 8th - 9th day of incubation (stages S34-S35) seemed to be a crucial period for embryos from this group.

Concluding, the embryos of miniature fowls seem to be less sensitive on manipulation and better tolerate culture in surrogated eggshells than broilers embryo therefore they can become comfortable experimental model in biology

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