Membrane-tropic effect of aurum and argentum nanoparticles on the intensity of oxidative processes in escherichia cells under the conditions of their lyophilization/ rehydration
M. Ye. Roman'ko
There was determined that lyophilization/ rehydration processes inhibit intensity of lipid peroxidation processes ((¹ 25, 20) and increase protein oxidation (¹ 25, 24, 20) in the membranes of E. coli rehydrative cells in comparison with the state of these processes in the native cell membranes (ð ? 0,05). At that in the cell membranes of experimental strains there was registered expressed inhibition of catalase activity, reduce of activity indices, that was at the average from 32,2 % to 77,9 %, and 91,9% increase of the level of total antioxidant activity.
Under the conditions of contact interaction with aurum nanoparticles (Au) with the size ~30 nm and ~45nm and argentum (Ag) with the size ~30 nm regulatory mechanisms of oxidative processes in E. coli cell membranes are characterized in total by interference of total antioxidant activity reactions, changes of catalase activity and inhibition of protein oxidation ketoderivatives creation, that may cause potential of investigated strains cells to their resistance at the influence of stress factors.
There was determined, that potential of own antioxidative system of E. coli lyophilized cell membranes, strains ¹ 20 and 24 was not enough for the maintenance of membrane lipid native structure and for the prevention of AOM influence even after incubation with Au nanoparticles with the size ~30 nm. Multidirectional changes of determinate characteristics in the E.coli membranes of different strains are the undoubted fact of individual specific character of nanopreparations' influence of the cell functional state in the bounds of one species. As a result of obtained data there was determined that an interaction of nanopreparations with different strains of E. coli cells there had not been registered dependence of the character of their effect from the selected range of concentrations.
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