Morphological and cytogenetical characteristics of porcine oocytes at parthenogenetic embryos produced in vitro depending on the conditions maturation
L. I. Ostapovets
The results of comparative analysis of morphological and cytogenetical parameters of porcine oocytes during their activating to the parthenogenetic development depending on the conditions maturation are presented in the article. It was determined, that the cultivation of porcine oocyte-cumulus complexes in medium TS 199 with supplements of 20 % estrous blood serum of cows positively influences on the meiotic maturation, increased the rate of oocyte, matured in vitro to metaphase II stage meiosis, comparative with the culturing in medium NCSU-23+ÁÑÀ. It was also shown that maturation of oocyte in medium TS 199 with supplements of 20 % estrous blood serum of cows positively influences on the cytoplasmic maturation, increased the cell-devision frequency of parthenogenetic activated porcine oocytes and parthenogenetic embryos produced cytoplasmic in vitro on the 5-16-cellular stage of development.
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