Evaluation of tolerance of crucian carp carassius auratus under effect of pollution using the battery of specific and non-specific biochemical marker

H. I. Falfushynska

To evaluate the adaptive ability of crucian carp from two sites in relatively clean (Z) and polluted (B) area, animals were treated by copper (0,005 and 0,05 mg l 1 Cu2+) and manganese (0,17 and 1,7 mg l 1 Mn2+) salts during 14 days. In the fish from B-group compared with Z-group the features of reside in toxic surrounding such as a higher level of metallothioneins (MTs) (by thiols measuring), lower metal-binding ability of MTs, and especially activity of superoxide dismutase isoforms were observed. Effects of metal ions in fish from both groups were enhanced of superoxide anion formation and activated of glutathione-S-transferase. The site- and metal-specific changes for animal protein carbonyls under exposure condition were shown. The enlargement of MTs contents were found only in the Z-group, while in animals from B-group some features of excess of MTs detoxification capacity were testified.

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