Superpxidedismutase isoforms at bull ejaculates incubation
N. V. Kuz'mina, D. D. Ostapiv, I. M. Yaremchuk
Isoenzyme superoxidedismutase spectrum in bulls' ejaculates incubation process was studied. It was established that 5 superoxidedismutase isoforms are characteristic for sperm and they differ by electrophoresis' activity and display intensity. SOD-isoforms spectrum changes in bulls' sperm incubation process: S1 and S2 isoforms (ð < 0,001) content probably increases with incubation time increase and S4 - to the contrary decreases (ð < 0,001). Strong direct correlation exists between incubation duration and S1 and S2 content, and inverse with S4 isoforms. With spermatozoa incubation time increase content of S1 and S2 isoforms SOD probably increases reaching it's maximum on the third day at spermatozoa survival more than 100 hours, and more than 100 hours - on the 4th. Content of S4-isoform SOD probably decreases (ð < 0,001) on the second day of incubation at spermatozoa survival less than 100 hours and at physiologic indices more than 100 hours on third. Between the survival of spermatozoa and maintenance of S1-, S2-izoforms strong direct correlation exists (?2S1=0,84-0,91, ?2S2=0,93-0,89), for S4-izoform - reverse (?2S4=0,90-0,85).
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