Lipoproteins bovine sperm under addingantioxidants in extender addition

S. Yo. Kava, I. M. Yaremchuk, D. D. Ostapiv

The formation of complex lipoproteins in sperm bulls under antioxidants extender addition was studied. Established that the experimental bulls characterized ejaculate volume 4,3 ± 0,18 ml concentration, 1,09 ± 0,11 x 109 / ml spermatozoa and their mobility 7,4 ± 0,16 points, containing protein-lipid complexes represented hilomikrons 26,9 ± 1,93 % and varying density lipoproteins: very low - 10,4 ± 0,44 %, low - 18,3 ± 1,84 %, high - 17,1 ± 1,09 % and very high - 26,8 ± 1,94 %. Dilution of ejaculates with lactoses-egg yolk-glycerin extender leads to the formation of macromolecules lipoprotein with low of electrophoresis mobility. Reduced form of glutathione and ascorbic acid in composition of ejaculate extender promote the formation of lipoprotein complexes with increasing size of their molecules and a lowers of electrophoresis mobility and provide protection from damage in sperm incubation processes.

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