Development of methods of solyubilization of viruses membranes and their application at making of blood preparations

T. V. Danysh

Basic theoretical and practical approaches in relation to a selection and use of detergents and organic solvents for the inactivation of lipid-enveloped membrane viruses at the receipt of pharmaceutical and veterinary preparations, their advantage, and failings are considered. The examples of application of chemical agents for effective inactivation of viruses of hepatitis B and C, and also HIV are resulted. The methods of separation of inactivation substances from protein preparations are described. The solvent-detergent method of viral inactivation is described. It is shown, that the method of chromatography with the using of macroporous silica sorbents allows to obtain protein preparations from plasma of blood with high efficiency and productivity without presence Triton X-100 and tri(n-butyl)phosphate - potential toxic agents in its composition.

Download full text in PDF format