USE OF GENETIC MARKERS IN STRATEGY OF COMING TO DECISION ABOUT BIOLOGICAL CONTROL OF GYPSY MOTH'S NUMBER

V. V. Oberemok

RAPD-PCR analysis and allozyme analysis created opportunity to reveal genetic difference between gypsy moth individuals correlated with susceptibility of insect to viral and bacterial infections. Use of RAPD-PCR analysis with primer OPA-14 led to find genetic markers of gypsy moth individuals related with intensity of development of viral infection in the host. RAPD аlleles approximately 150, 430, 850 and 1000 b.p. long are the markers of resistance аnd RAPD alleles approximately 330 and 560 b.p. long are the markers of susceptibility of insect to virus. With the help of allozyme analysis it was found that gypsy moth individuals heterozygotic to Est-5 locus are in average more resistant to bacterial infection than homozygotic. On the basis of genetic markers of susceptibility of gypsy moth to viral preparation "VIRIN-NSH" and bacterial preparation В3 variant of genetic approach in strategy of coming to decision about biological control of gypsy moth's number was suggested.

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