BUSULFAN USING FOR BIRDS' GONAD STERILIZATION

M. T. Tagirov, A. B. Artemenko, S. N. Tkachenko, L. V. Terecshenko, A. V. Terecshenko

Effectiveness of busulfan treatment as a chemical agent for birds' gonad sterilization was specified in two experiments. Busulfan (300 ng/egg) is injected into subgerminal cavity after preliminary egg incubation at 37,8 °С for 8-10 hr for the production of chick germ line chimeras. Before the injection of donor cells, for inactivation of busulfan action the eggs are incubated at 31-32 °С for 24 hr.

For sterilization of chick gonads in gonad transplantation experiments three-fold busulfan injection of 70 ?g/egg every 24 hr of incubation or single injection of 250 ?g/egg of busulfan into fresh laid eggs is used, which ensure 46,2 % of gonad sterilization.

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