THE EFFECT OF HORMONE STIMULATION ON MORPHOLOGICAL AND ELECTRIC PARAMETERS OF MURINE OOCYTES

E. I. Smolyaninova, V. A. Shigimaga, A. A. Kolesnikova

The effect of gonadotrophin administration in mouse females to induce superovulation on morphologic and electric oocyte parameters is investigated. Hormone stimulation was carried out according three schemes: 1) GPSM+hLG; 2) GPSM; 3). Using the method of electroporation the specific electric conductivities of oocytes that were obtained as a result of superovulation and spontaneous ovulation were determined. It is shown that the whole pool of oocytes that were obtained after superovulation under schemes1 and 2 consists of two groups of oocytes that don't differ each from other morphologically but differ by their electric parameters and resistance to electric breakdown. It is shown that hormonal stimulation in mice leads to morphological injury of oocytes in comparison with oocytes obtained after spontaneous ovulation. This study shows that folliculostimulate hormone affects essentially the quality of oocytes. As a result of administrating with GPSM the oocytes of different developmental functional potential are able to ovulate.

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