PARENTAGE VERIFICATION OF UKRAINIAN RIDER HORSE BREED USING MICROSATELLITES DNA MARKERS

V. Spyrydonov, A. Sheliov, S. Melnychuk, T. Ilnytska

The present study was to evaluate a parentage verification system for Ukrainian Rider horse breed using 14 DNA markers. A total number of 40 Ukrainian Rider horse including 13 foals for parentage verification were genotyped. The microsatellite loci showed extensive polymorphism with allele numbers ranging from 5 to 12 and polymorphism information content (PIC) values in the range 0,482-0,879. The analysis of these loci also revealed that they had a 99,99993 % combined probability of exclusion (CPE) of false parentage. This study suggests that the DNA typing method has high potential for parentage testing and individual identification of Ukrainian Rider horse breed.

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