COMPARATIVE MAPPING OF THE UNSTABLE CGG TANDEM REPEATS OF THE HUMAN FRM1 GENE IN FARM ANIMALS

U. Kaczor, B. Danielak-Czech, M. Sharan

Based on in situ PCR technique with primers flanking CGG repeats located in the 5'UTR region of the human FRM1 mRNA, the trinucleotide repeats of the FRM1 gene have been mapped to the structurally unstable X chromosome regions of cattle - BTA Xp13, sheep - OAR Xq22, goats - CHI Xq22 and pigs - Xq26. The experiment confirmed homology of regulatory sequences in CGG region of FRM1 gene and conservation of the corresponding unstable X chromosome regions in mammals. These results can be applied in comparative evolutionary studies and make it possible to ascertain the relationships between region-specific X chromosome instability and phenotypic effects in farm animals.

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