Application of classical-pcr, nested-pcr and seminested-pcr in identification of helicobacter species colonizing gastric mucosa in dogs with gastritis

Krzysztof Kubiak, Marcin Jankowski, Jolanta Spu?ak, Kamila Gli?ska-Suchocka, Jacek Ska?a*, Gra?yna Go?ciniak**, J?zef Nicpo?

Colonization of dogs' gastric mucosa with Helicobacter spp. is common. Based on the conducted research it was demonstrated that microorganisms of Helicobacter morphology colonizing the gastric mucosa of dogs (GHLO - gastric Helicobacter-like organisms) included: Helicobacter felis, Helicobacter heilmannii, Helicobacter bizzozeronii, Helicobacter bilis, Flexispira rappinii and Helicobacter salomonis. These bacteria were found in the stomach of healthy animals and the stomach affected by the disease process. They were observed in the cardia, the fundus, the corpus as well as the pylorus of this organ.

In the diagnosis of the gastric mucosa infections with Helicobacter spp. in humans and animals non-invasive and invasive methods are applied. The non-invasive methods include: a breath urease test and serological examinations. The invasive methods are based on the laboratory examination of the gastric mucosa bioptates and they include: a rapid urease test, direct bacteriological preparation, microbiological culture examinations, histopathological examinations (a light and electron microscope) and examinations using polymerase chain reaction (PCR).

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