To the question about possible correlation between release of k+ ions and development of hemolytic damage of mammalian erythrocytes under hypertonic cryohemolysis

N. M. Shpakova, S. S. Ershov, O. E. Nipot

The paper covers the studies of release of potassium ions from mammalian erythrocytes in hypertonic media under varying the temperature and concentrations of salt and cell hemolysis at their further cooling down to 0?C. It has been shown that release of K+ ions from human and animal erythrocytes increases with salt concentration and and arrives at practically a maximal value in solution, containing 2,0 M of NaCl, while dependences erythrocytes hemolysis of different mammals types differ. For the red blood cells of all investigated types of mammals there is an increase of level of cryohemolysis at the increase of temperature of incubation, but increase of level of release of potassium ions characteristically only for erythrocytes of man, horse and rabbit. Potassium release is assumed as not single condition determining the character of development of hemolytic damage of cell under cryohemolysis.

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